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Image Search Results
Journal: Virology
Article Title: Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells
doi: 10.1016/j.virol.2022.01.014
Figure Lengend Snippet: SARS-CoV-2-induced cytopathic effect and viral detection by immunohistochemistry in cells expressing human ACE2 and TMPRSS2 . Vero, DF1, DF1 expressing both human ACE2 and TMPRSS2 (++), MDCK, and MDCK expressing both human ACE2 and TMPRSS2 (++) were grown at 37C in 5% CO 2 on glass chamber slides. Cells were inoculated with SARS-CoV-2 at MOI of 1. At 48 h post inoculation monolayers were examined for cytopathic effect and detection of virus with rabbit monoclonal antibodies against SARS-CoV-2 spike and nucleoprotein. Cells were washed 3 times with PBS and incubated in the secondary antibody, goat anti-rabbit IgG H&L (Alexa Fluor® 555) for 1 h at room temperature. Cells were then washed counterstained with DAPI. Immunofluorescence was visualized with an EVOS 5000.
Article Snippet: Primary antibodies against SARS-CoV-2 included
Techniques: Immunohistochemistry, Expressing, Virus, Bioprocessing, Incubation, Immunofluorescence
Journal: Virology
Article Title: Development of an in vitro model for animal species susceptibility to SARS-CoV-2 replication based on expression of ACE2 and TMPRSS2 in avian cells
doi: 10.1016/j.virol.2022.01.014
Figure Lengend Snippet: SARS-CoV-2 induced cytopathic effect and viral detection by immunohistochemistry in DF1 cells expressing animal species ACE2 and TMPRSS2 . DF1 cells expressing animal ACE2 and TMPRSS2 were grown at 37C in 5% CO 2 on glass chamber slides. Cells were inoculated with SARS-CoV-2 at MOI of 1. At 48 h post inoculation monolayers were examined for cytopathic effect and detection of virus with rabbit monoclonal antibodies against SARS-CoV-2 spike and nucleoprotein. Cells were washed 3 times with PBS and incubated in the secondary antibody, goat anti-rabbit IgG H&L (Alexa Fluor® 555) for 1 h at room temperature. Cells were then washed counterstained with DAPI. Immunofluorescence was visualized with an EVOS 5000.
Article Snippet: Primary antibodies against SARS-CoV-2 included
Techniques: Immunohistochemistry, Expressing, Virus, Bioprocessing, Incubation, Immunofluorescence